Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 8,560 results for: Dos

Clear keywords
Showing: 2461 - 2480 of 8560 results

  6. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61362-plasmid-map-sequence-id-99796

    Enlarge

  7. pMIA3-Pansio

    Plasmid
    #214445
    Purpose
    Expresses eSpCas9 and gRNA targeting Pansio-1 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214445-plasmid-map-sequence-id-433843

    Enlarge

  8. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

    Enlarge

  9. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Publication
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141250-plasmid-map-sequence-id-308875

    Enlarge

  17. EF1α-eGFP-Z1

    Plasmid
    #135612
    Purpose
    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Publication
    Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Expression
    Mammalian
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135612-plasmid-map-sequence-id-269403

    Enlarge

  18. pTF102

    Plasmid
    #134774
    Purpose
    A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.
    Depositor
    Kan Wang
    Publication
    Frame et al Plant Physiol. 2002 May;129(1):13-22. do
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    134774-plasmid-map-sequence-id-263749

    Enlarge

Showing: 2461 - 2480 of 8560 results