We narrowed to 14,071 results for: cas9

1. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEf1a-Dn29-dCas9-P2A-GFP

   Plasmid

   #247155

   Purpose

   Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   Dn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEf1a-superDn29-dCas9-P2A-GFP

   Plasmid

   #247156

   Purpose

   Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   superDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEf1a-hifiDn29-dCas9-P2A-GFP

   Plasmid

   #247158

   Purpose

   Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEf1a-goldDn29-dCas9-P2A-GFP

   Plasmid

   #247157

   Purpose

   Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   goldDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYJ28-AAVS1-XLone-dCas9-p300core

   Plasmid

   #131071

   Purpose

   Donor plasmid of targeted inducible-dCAS9-P300core knockin

   Depositor

   Xiaojun Lian

   Insert

   dCas9-p300core
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE3G-ZIM3-Cas9-P2A-GFP

   Plasmid

   #239605

   Purpose

   Doxycycline inducible CRISPRgenee construct

   Depositor

   Philipp Rathert

   Article

   Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

   Insert

   ZIM3 KRAB domain (ZIM3 )
   

   Use

   CRISPR and Lentiviral

   Promoter

   TRE3G

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1as-ZIM3-Cas9-P2A-GFP

   Plasmid

   #239603

   Purpose

   Constitutive active CRISPRgenee construct

   Depositor

   Philipp Rathert

   Article

   Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

   Insert

   ZIM3 KRAB domain (ZIM3 )
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro

   Plasmid

   #226427

   Purpose

   PiggyBac transposon vector containing a dox inducible mutant p300 core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP reporter. Hygromycin selection marker.

   Depositor

   Alon Goren

   Inserts

   Mutant p300 core-dCas9-EGFP fusion protein
   rtTA3-IRES-Hygro
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   HA tag, Mutant p300 core, and P2A-EGFP

   Expression

   Mammalian

   Mutation

   p300 core mutation (D1399Y)

   Promoter

   Dox inducible minimal CMV and UbC Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

    Plasmid

    #231998

    Purpose

    Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B-targeted sgRNA (CHMP2B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

11. Lenti-E14-ABE-Cas9n-puro

    Plasmid

    #226588

    Purpose

    Express E14-ABE in mammalian cells

    Depositor

    Gang Wang

    Article

    Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

    Insert

    E14-ABE
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. nzCas9n-GSG-linker-P2A-mNeongreen

    Plasmid

    #241189

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid

    #210391

    Purpose

    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

    Depositor

    Benjamin Kleinstiver

    Article

    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

    Insert

    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CamKII promoter for Cre, human U6 promoter for Sp…

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

    Plasmid

    #236743

    Purpose

    Constitutive expression of Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
    EF1a-hygro-WPRE
    

    Expression

    Mammalian

    Promoter

    CAG and EF1a

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 Cas9-P2A-dTomato-PS9

    Plasmid

    #231911

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 Cas9-P2A-dTomato-T20

    Plasmid

    #231912

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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