We narrowed to 2,757 results for: fas

1. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt1(E17K)

   Plasmid

   #86563

   Purpose

   Expresses human full-length Akt1(E17K) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   E17K mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn FLEx-loxp hM4Di-mCherry

   Plasmid

   #161581

   Purpose

   Expression of hM4Di-mCherry in a cre-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   hSyn

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

   Plasmid

   #184633

   Purpose

   Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Aldh1a1 (Aldh1a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBD-Akt1(K14A)

   Plasmid

   #86562

   Purpose

   Expresses human full-length Akt1(K14A) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   K14A mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIFR-5

   Plasmid

   #233294

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   p14G-BCD2

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. UBQ10pro-nlsABACUS2-400n-NosT

   Plasmid

   #203728

   Purpose

   Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

   Depositor

   Alexander Jones

   Article

   Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

   Insert

   nlsABACUS2-400n
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10pro 1500bp

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN_C74_eMagAF_V5_NES

    Plasmid

    #235636

    Purpose

    Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    eMagAF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 74-321

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGEN_N256_eMagBF_V5_NES

    Plasmid

    #235630

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-256, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-256

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGEN_NES_V5_eMagAF_C233

    Plasmid

    #235629

    Purpose

    Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    NES-V5-eMagAF

    Expression

    Mammalian

    Mutation

    residues 233-321

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGEN_NES_V5_eMagAF_C141

    Plasmid

    #235621

    Purpose

    Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    NES-V5-eMagAF

    Expression

    Mammalian

    Mutation

    residues 141-321

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGEN_NES_V5_eMagAF_C222

    Plasmid

    #235627

    Purpose

    Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    NES-V5-eMagAF

    Expression

    Mammalian

    Mutation

    residues 222-321

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGEN_NES_V5_eMagAF_C196

    Plasmid

    #235625

    Purpose

    Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    NES-V5-eMagAF

    Expression

    Mammalian

    Mutation

    residues 196-321

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGEN_N232_eMagBF_V5_NES

    Plasmid

    #235628

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-232, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-232

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGEN_MyrPalm_NES_V5_eMagAF_C74

    Plasmid

    #235639

    Purpose

    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    MyrPalm-NES-V5-eMagAF

    Expression

    Mammalian

    Mutation

    residues 74-321

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN_V5_eMagAF_C74_cMycNLS

    Plasmid

    #235640

    Purpose

    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    V5-eMagAF and cMycNLS

    Expression

    Mammalian

    Mutation

    residues 74-321

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGEN_N140_eMagBF_V5_NES

    Plasmid

    #235620

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-140, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-140

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGEN_NES_V5_eMagAF_C74_Sec61b

    Plasmid

    #235637

    Purpose

    Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi C-terminal fragment
    

    Use

    Tags

    NES-V5-eMagAF and Sec61b

    Expression

    Mammalian

    Mutation

    residues 74-321

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge