We narrowed to 7,494 results for: tet

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Mutation

mouse-human fusion

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6 asyn A30P

Plasmid

#105729

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Mammalian

Mutation

A30P

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1-mCherry-Puro

Plasmid

#193763

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

WT

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔPIP-mCherry-Puro

Plasmid

#193765

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔPIP-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

PIP degron removed (a.a.1-19 removed)

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

Plasmid

#69583

Purpose

Expresses p53 tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔCy-mCherry-Puro

Plasmid

#193764

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔCy-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(1-303)IntN

Plasmid

#200312

Purpose

Bacterial expression of P53 NTAD-DBD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Tags

His6GB1tev and designed intein N

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS423-mitoT

Plasmid

#174525

Purpose

Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

Yeast mitochondrial intermembrane tether

Use

Synthetic Biology

Tags

eGFP

Expression

Yeast

Promoter

MET25

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p4949 pLPCX-His-Xpress NLS hBrd4 CTD

Plasmid

#14459

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Use

Retroviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pX131

Plasmid

#167149

Purpose

MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

4'-phosphopantetheinyl transferase, npgA

Use

Synthetic Biology

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

Plasmid

#174647

Purpose

Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

CMV (with TetOn)

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

Plasmid

#14442

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
GFP-cCGNL1-HA

Plasmid

#236517

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Tags

GFP and HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1

Plasmid

#236519

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (Cgnl1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-Myc

Plasmid

#236506

Purpose

For expression of CGN (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT to BioID-nDIC2-3xFlag

Plasmid

#232418

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-cDIC2-3xFlag

Plasmid

#232416

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 1-355

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 356-652

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C 15A

Plasmid

#113007

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K

Plasmid

#89380

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl F

Plasmid

#89375

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G

Plasmid

#89376

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl J

Plasmid

#89379

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl E

Plasmid

#89374

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl I

Plasmid

#89378

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl H

Plasmid

#89377

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TRIP8b (exon 1b) [N212A/34R] (Recombinant Antibody)

Antibody

#209600-rAb

Purpose

Anti-TRIP8b (exon 1b) (Mouse) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Aug. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits
pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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