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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 128,572 results for: CIL

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Showing: 2481 - 2500 of 128572 results

  1. pRK5-myc-RhoA-T19N

    Plasmid
    #12963
    Depositor
    Gary Bokoch
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RhoA DN (RHOA Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T19N Dominant negative
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12963-plasmid-map-sequence-id-223086

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  2. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid
    #71707
    Purpose
    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
    Depositor
    Giulio Draetta
    Publication
    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    Use
    CRISPR
    Tags
    3xFLAG and hGEM(1/110)
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71707-plasmid-map-sequence-id-424083

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  3. pGFP FAK

    Plasmid
    #50515
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Publication
    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    protein tyrosine kinase
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50515-plasmid-map-sequence-id-352443

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  8. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid
    #107738
    Purpose
    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
    Depositor
    Rylan Larsen
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    Cre, dTomato
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107738-plasmid-map-sequence-id-207220

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  11. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid
    #73499
    Purpose
    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73499-plasmid-map-sequence-id-364405

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  12. pCLIP-tag (m) Vector

    Plasmid
    #101136
    Purpose
    Expression of protein of interest with CLIP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    CLIP-tag (CLIP10)
    Expression
    Mammalian
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101136-plasmid-map-sequence-id-194177

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  13. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Publication
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140095-plasmid-map-sequence-id-292013

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  17. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Publication
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LDLR (LDLR Human)
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98184-plasmid-map-sequence-id-490370

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Showing: 2481 - 2500 of 128572 results