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pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PARP1
Plasmid#215136PurposeExpresses mCherry-PARP1 in mammalian cells from a retroviral vector.DepositorInsertPARP1 (PARP1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_M18
Plasmid#82357PurposeExpression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
Plasmid#207861PurposeAAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-human cGAS-HA
Plasmid#186896PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS with C-terminal HA tag (Adamts1 Human)
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH2
Plasmid#215121PurposeExpresses AcGFP-MSH2 in mammalian cells from a retroviral vector.DepositorInsertMSH2 (MSH2 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a1SE
Plasmid#49168PurposepHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
UseTagsmyc and pHGFP (pHIuorin)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRTagsExpressionMammalianMutationPromoterPgkAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-928-pGex2TK
Plasmid#21749DepositorInsertRb (RB1 Human)
UseTagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928 of wt Rb …PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 RD/LD
Plasmid#186476PurposeExpression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH6
Plasmid#215123PurposeExpresses AcGFP-MSH6 in mammalian cells from a retroviral vector.DepositorInsertMSH6 (MSH6 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only
