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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pRSETB

Plasmid

#55775

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

6xHis and msEGFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pBP-TL4

Plasmid

#72988

Purpose

RBS_H5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

H5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL8

Plasmid

#72992

Purpose

RBS_L1

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-L1U1H09

Plasmid

#73008

Purpose

L1U1H09 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1U1H09 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pMulti-sgRNA-LacZ

Plasmid

#99913

Purpose

Receptor plasmid for the assembly of multiple sgRNAs

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCcmO-StrepII-PDT

Plasmid

#165584

Purpose

Native CcmO fusion with a StrepII tag and PDT

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

CcmO-PDT

Tags

Protein degradation tag

Expression

Bacterial

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-PcPT-ximD-ximE

Plasmid

#172654

Purpose

Production of decursinol in E. coli

Depositor

Minjuan Xu

Article

Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

Insert

PcPT, XimD and XimE

Use

Synthetic Biology

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMM763

Plasmid

#68900

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Mutation

G1104E

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCG-D-SpyTag-His6-E

Plasmid

#87375

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Tags

His6 tag

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-YRNRV

Plasmid

#63625

Purpose

N4-R5-YRNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-YRNRV RNAP

Mutation

N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 1

Plasmid

#30159

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 1

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Article

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS15c-RFP

Plasmid

#126198

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbE12k-RFP

Plasmid

#126151

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS20k-RFP

Plasmid

#126196

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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