We narrowed to 4,387 results for: 187

1. JBEI-3716

   Plasmid

   #100169

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   codon-optimized MK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV FLIP p53

   Plasmid

   #19745

   Depositor

   Richard Hynes

   Article

   Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   oligo targeting p53 (Trp53 Mouse)
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCerulean3-Alpha-5-Integrin-12

   Plasmid

   #55401

   Purpose

   Localization: Focal Adhesions, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Alpha-5 Integrin (ITGA5 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEntry_HCoV-229E-nsp5

   Plasmid

   #168954

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-229E-nsp5 (HCoV229Egp1 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mutpRL-TK 3xUTR

   Plasmid

   #40758

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   3 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAcGP67A-hBAI3-HormR-GAIN

   Plasmid

   #37848

   Depositor

   Axel Brunger

   Article

   Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

   Insert

   BAI3 (BAI3 Human)
   

   Use

   Baculovirus transfer vectors

   Tags

   8xHIS

   Expression

   Mutation

   HormR and GAIN domains only (aa498–868

   Promoter

   Polyhedrin

   Available since

   July 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PKM-IRES-hrGFP

   Plasmid

   #73405

   Purpose

   Mammalian expression of mouse PKM and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKM (Prkcz Mouse)
   

   Use

   Tags

   3xFLAG and pIRES-hrGFP-2a

   Expression

   Mammalian

   Mutation

   truncated, constitutively active form

   Promoter

   CMV

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. EGFP-UVRAG S498A

   Plasmid

   #86741

   Purpose

   N-terminal EGFP-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human UVRAG (UVRAG Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S498A

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-UVRAG S498A

    Plasmid

    #86744

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAT-mWasabi-G

    Plasmid

    #164170

    Purpose

    Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

    Plasmid

    #172103

    Purpose

    Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. myc-UVRAG S498A

    Plasmid

    #86737

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19 PKC epsilon

    Plasmid

    #8442

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

    Insert

    Mouse Protein Kinase C epsilon (Prkce Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGL2 p15 4xSBR2 MutSBE

    Plasmid

    #15707

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    p15 4xSBR2 (CDKN2B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutant SBE (Smad binding element)

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

17. b2-TS5

    Plasmid

    #103878

    Purpose

    full-length human b2 with tension sensor at b2_743

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_743 (ITGB2 Human)
    

    Use

    Tags

    tension sensor module

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGlomyc-Frat1

    Plasmid

    #124499

    Purpose

    Expresses myc-tagged mouse FRAT1 in mammalian cells (N-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Frat1 (Frat1 Mouse)
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGlomyc-Frat2

    Plasmid

    #124500

    Purpose

    Expresses myc-tagged mouse FRAT2 in mammalian cells (N-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Frat2 (Frat2 Mouse)
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. myc-UVRAG S498D

    Plasmid

    #86739

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S498D

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge