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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2521 - 2540 of 2874 results

  1. pMesh1

    Plasmid
    #175594
    Purpose
    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175594-plasmid-map-sequence-id-348364

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  2. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available Since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

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  6. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  13. pGBW-m4136464

    Plasmid
    #149534
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149534-plasmid-map-sequence-id-307562

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  15. pGBW-m4136461

    Plasmid
    #149538
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149538-plasmid-map-sequence-id-307561

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  16. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145670-plasmid-map-sequence-id-297837

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  19. pGL2

    Plasmid
    #48742
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +386)
    Use
    Luciferase
    Mutation
    truncated promoter region (see pGL6)
    Available Since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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Showing: 2521 - 2540 of 2874 results