We narrowed to 11,554 results for: Vars;

1. pET15b I-SceI (Ctermhis -MH)

   Plasmid

   #195613

   Purpose

   Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1.2-Hygro

   Plasmid

   #162741

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-R4-uAb

   Plasmid

   #101801

   Purpose

   Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

   Depositor

   Matthew DeLisa

   Article

   Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

   Insert

   R4-uAb
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1.2-Neo

   Plasmid

   #162739

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO Elongation factor 1A

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQP-2473

   Plasmid

   #138976

   Purpose

   Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS17: pTwinStrep-SUMO-AvTnsD

   Plasmid

   #168150

   Purpose

   AvTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsD
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAS-MEYFP

   Plasmid

   #86372

   Purpose

   membrane targeted EYFP expression in zebrafish

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   EYFP
   

   Use

   Zebrafish

   Tags

   GAP43 membrane targeting

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW194b63

   Plasmid

   #136930

   Purpose

   Directed evolution of SpCas10

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   npuC-gIII(C)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ211_WT_hi

    Plasmid

    #128850

    Purpose

    High copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ40-GFP-iBox-PAK4cat

    Plasmid

    #190179

    Purpose

    expresses GFP-tagged iBox-PAK4cat

    Depositor

    Bianxiao Cui

    Article

    Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

    Insert

    GFP-iBox-PAK4cat
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL13-MgtC bb118

    Plasmid

    #158982

    Purpose

    Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mgtC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1435

    Plasmid

    #111439

    Purpose

    Solo vector pV1382 + sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    A12G

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKSB-611U6-sgRNA3 AGAG AACA

    Plasmid

    #173673

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNAP-EF(N)-10nmSAH-GFP(1-10)

    Plasmid

    #61660

    Purpose

    10nm SAH scaffold with EF Hand and split GFP attachment sites

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    SNAP-EF(N)-10nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Endosomes-13

    Plasmid

    #56433

    Purpose

    Localization: Endosomes, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pSV2-c-erbB2

    Plasmid

    #16250

    Depositor

    Mien-Chie Hung

    Article

    Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

    Insert

    c-erbB2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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