We narrowed to 13,608 results for: crispr cas9

1. pT7-AGG-SpCas9

   Plasmid

   #214603

   Purpose

   encode SpCas9; in vitro transcribed modified RNA vector

   Depositor

   Yuxuan Guo

   Article

   Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

   Insert

   SpCas9
   

   Use

   CRISPR and Unspecified

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2_NOP1gRNA-Cas9-CsA

   Plasmid

   #136139

   Purpose

   Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-spCas9

   Plasmid

   #231404

   Purpose

   Expresses spCas9 from hSyn promoter

   Depositor

   Larry Young, Arjen Boender

   Article

   Schimmer et al bioRxiv 2024.07.15.603543; doi: https://

   Insert

   hSYN promoter (RIC8B Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   Changed RSV promoter to hSYN promoter

   Promoter

   hSYN

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187960

   Purpose

   AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

   Plasmid

   #218710

   Purpose

   AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47869

   Purpose

   All-in-one plasmid targeting tdTomato

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   tdTomato crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

   Plasmid

   #127560

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR

   Expression

   Insect

   Mutation

   EGFP-D102G see depositor comments below

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

    Plasmid

    #227009

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid

    #227010

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

    Plasmid

    #227011

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

    Plasmid

    #227012

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-DIO-Cas9

    Plasmid

    #166601

    Purpose

    Encodes Cre-dependent Cas9 under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    Cas9
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hRad51(A89E)–Cas9(D10A)

    Plasmid

    #125563

    Purpose

    Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A89E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. hRad51(K133R)–Cas9(D10A)

    Plasmid

    #125568

    Purpose

    Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(K133R)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Mutation

    Enzymatically dead Cas9

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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