We narrowed to 32,159 results for: uros

1. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

   Plasmid

   #223160

   Purpose

   Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   dGFP-P2A-NanoLuc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-TetCMV-puro-mCherry-PA-Rac1

   Plasmid

   #22035

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   Tet-CMV

   Available Since

   Sept. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-TRE3G-FNLS-PGK-Puro

   Plasmid

   #110847

   Purpose

   Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   TRE3G

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SauriCas9-KKH-Puro

   Plasmid

   #135966

   Purpose

   Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

   Plasmid

   #221551

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human 3' HP1a AID GFP PuroR

   Plasmid

   #127906

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   HP1 (CBX5 Mustard Weed, Human)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Hp1a

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMLV-CAG-TVA-IRES-Puro

   Plasmid

   #172366

   Purpose

   Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + Puro
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Puro-T2A-mCitrine-hSmad2-donor

   Plasmid

   #203663

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   Smad2
   

   Tags

   mCitrine

   Expression

   Mammalian

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMJA285= pSin-TCRab-CMVp-Puro

   Plasmid

   #116875

   Purpose

   stable TCR expression in human T cells

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   TCR (TRAV10, TRBV25-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   deletion of EF1-a promotor

   Promoter

   CMV Promotor

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE puro mouse IRS-2 myc

    Plasmid

    #11373

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

    Plasmid

    #172611

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDKN1B (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid

    #214125

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-TET
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

    Plasmid

    #165082

    Purpose

    gRNA 1 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti c-MYC-DDK-Puro GFP

    Plasmid

    #123299

    Purpose

    Mammalian expression of GFP C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lv EF1a SS18-Frb PGK Puro

    Plasmid

    #102811

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    SS18-Frb
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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