We narrowed to 152,655 results for: des

1. pAN2981

   Plasmid

   #127246

   Purpose

   Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR-PCBP1

   Plasmid

   #16177

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   Poly(rC) binding protein 1 (PCBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pRSET a MatryoshCaMP6s

   Plasmid

   #100019

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR223-GFP

   Plasmid

   #198589

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   GFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   None (wildtype)

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAd-Track Flag GCN5 Y621A/P622A

   Plasmid

   #14425

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   GCN5 Y621A/P622A (KAT2A Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Y621A, P622A. Catalytically inactive acetyltransf…

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

6. FUW-mTurquoise

   Plasmid

   #193092

   Purpose

   constitutive expression of mTurquoise in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mTurquoise
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B1 Integrin-GFP

   Plasmid

   #186138

   Purpose

   For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Tags

   MBP and mEGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR223-BFRF3

   Plasmid

   #195778

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BFRF3

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BFRF3
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3XFlag-Mettl4

   Plasmid

   #86665

   Purpose

   Expresses murine Mettl4 containing N-terminal 3XFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Mettl4 (Mettl4 Mouse)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3xFlag-Alkbh4

    Plasmid

    #105240

    Purpose

    Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Alkbh4 (Alkbh4 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR223-BKRF3

    Plasmid

    #195790

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BKRF3

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BKRF3
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSF2C-A-RFP

    Plasmid

    #172432

    Purpose

    StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR223-BGLF2

    Plasmid

    #195800

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BGLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BGLF2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EYFP-IKB

    Plasmid

    #111194

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    IKB-alpha
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-PKCG

    Plasmid

    #16180

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    PRKC gamma (PRKCG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pBacPAK p110a RBD DM

    Plasmid

    #55723

    Purpose

    His3 GST tagged RBD double mutant p110 alpha T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    His3 GST

    Expression

    Mutation

    T207D, K227A

    Promoter

    polyhedrin promoter

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

18. NKP64 GFP (1007)

    Plasmid

    #62020

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. CSII-IDR2-owlTRIMCyp-FOS

    Plasmid

    #79067

    Purpose

    Express owl monkey TRIMCyp in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5Cyp (owl monkey)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CP1028-ABE8e-dimer

    Plasmid

    #138498

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEasyG3_mic

    Plasmid

    #184920

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMXs_FLAG-SFXN5

    Plasmid

    #110639

    Purpose

    Retroviral expression of Flag-SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. DDX5 D248N V5

    Plasmid

    #199577

    Purpose

    Expresses helicase dead DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Bxb1-ASAP2f

    Plasmid

    #183757

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJEP301-pAAV-CMV-MCS3-pA

    Plasmid

    #113676

    Purpose

    CMV driven Multi Cloning Site-3

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PXPR007 sgGFP-2

    Plasmid

    #74961

    Purpose

    Cas9 + sgGFP-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEmerald-H2B-6

    Plasmid

    #54111

    Purpose

    Localization: Nucleus/Histones, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

31. EYFP-NIK

    Plasmid

    #111208

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    NIK (MAP3K14 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs_SFXN5

    Plasmid

    #110640

    Purpose

    Retroviral expression of SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEasyG3_hph

    Plasmid

    #184919

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXs_FLAG-MFT

    Plasmid

    #136371

    Purpose

    Expression in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MFT (SLC25A32 Human)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEmerald-PDHA1-N-10

    Plasmid

    #54225

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

36. p414GPD-3xFLAG-Nop4 WT

    Plasmid

    #169261

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 WT (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ST-SBP-EGFP_puromycin

    Plasmid

    #65270

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-P1-N-miRFP670-C

    Plasmid

    #159438

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-P1-N-EGFP-C

    Plasmid

    #159436

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPPAT-2XStrep

    Plasmid

    #125620

    Purpose

    expresses human PPAT protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge