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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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NPC1 His6 EGFP

Plasmid

#53521

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Tags

EGFP and His6

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-H2B-SunTag24x_v1

Plasmid

#60911

Purpose

Enocdes a histone H2B fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

H2B

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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476 p5E-mcs

Plasmid

#26029

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Type

Empty backbone

Use

Entry vector

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1+SKL

Plasmid

#53450

Purpose

expresses GFP targeted for localization in peroxisomes

Depositor

Jay Brenman

Article

Sexton et al Int J High Throughput Screen. 2010 Jul;2

Insert

SKL amino acids

Tags

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only
pOPINF

Plasmid

#26042

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His3C-lacZ

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRsetB-His7tag-Peredox-mCherry

Plasmid

#32382

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

His7-Peredox-mCherry

Tags

His7tag

Expression

Bacterial

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Tags

GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pTK91_GFP-MRLC2

Plasmid

#46355

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

MRLC2 (MYL12B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-R3A

Plasmid

#8991

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry-NLS

Plasmid

#32384

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Rab5a-7

Plasmid

#55322

Purpose

Localization: Ras GTPase, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Rab5a

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSG5-BSLF1

Plasmid

#69834

Purpose

Mammalian expression vector for Epstein-Barr virus primase BSLF1

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BSLF1

Expression

Mammalian

Mutation

S2F

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Article

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Available Since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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