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pCMV-FLAG TIF1gamma M (456-887)

Plasmid

#15735

Depositor

Joan Massague

Publication

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma M (TRIM33 Human)

Tags

Flag

Expression

Mammalian

Mutation

Middle region (amino acids 456-887).

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC18-mini-Tn7T-Gm-GW

Plasmid

#64961

Purpose

mini-Tn7 Gateway destination vector

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFCM1

Plasmid

#64948

Purpose

source of CmR FRT cassette

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-cat-FRT

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p144 pSV-EGFP/beta-gal

Plasmid

#8386

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 NL

Plasmid

#12632

Depositor

Rik Derynck

Publication

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 NL (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-L segment of Smad3 (aa 1-211)

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
FRZBA-c400

Plasmid

#205109

Purpose

FRZB protein expression

Depositor

Paul Brennan

Insert

FRZB (FRZB Human)

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-SQSTM1

Plasmid

#223715

Purpose

Expresses Flag-tagged SQSTM1

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Publication

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Expression

Mammalian

Promoter

5xUAS and PGK

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SNRPA_Full-length_pGEX

Plasmid

#135134

Purpose

Recombinant expression of SNRPA with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SNRPA (SNRPA Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Feb. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Publication

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Tags

6xHis and MBP

Expression

Bacterial

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRS402-GPD-GFP(S65T)

Plasmid

#85190

Purpose

Integrating plasmid, GFP(S65T) under GPD promoter

Depositor

David Bartel

Publication

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

EGFP

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

GPD

Available since

Dec. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN G129E

Plasmid

#50523

Depositor

Kenneth Yamada

Publication

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

G129E

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-deltaHeat

Plasmid

#25068

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBTBX-1

Plasmid

#26067

Depositor

Ryan Gill

Publication

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pBTBXh-4

Plasmid

#26080

Depositor

Ryan Gill

Publication

Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

Type

Empty backbone

Tags

His

Expression

Bacterial

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pEF073 MBP-Axin-His

Plasmid

#154011

Purpose

Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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