We narrowed to 6,675 results for: proc
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Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
UseTagsGSTExpressionBacterialMutationS72TPromoterT7Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-mRFP1
Plasmid#112914PurposeRetrovirus expressing mRFP1 with BBSI cloning sites for sgRNADepositorInsertmRFP1
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoiseExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationS727APromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Zeo
Plasmid#25736PurposeLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-STOP
Plasmid#60077PurposeN-terminal GFP tagged CYLD with stop codon, driven by CMV promoterDepositorInsertCylindromatosis (CYLD Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLostfoX-Kan
Plasmid#99865PurposeTfoX induction vector for chitin-pathway transformation (KanR)DepositorInserttfoXVF
UseV. fischeri suicide cloning vector, cmr, tcrTagsExpressionMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-REVERBA-mScarlet-NLS-PEST
Plasmid#190658PurposeFluorescent reporter for circadian rhythmsDepositorInsertReverba-mScarlet (Nr1d1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS-RED-C1-Beclin1
Plasmid#24405DepositorInsertBeclin1 (BECN1 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2
Plasmid#25031DepositorInsertDCP2 (DCP2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3
Plasmid#48743PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1060)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL005635 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-NKX2-8
Plasmid#75080Purposeretroviral vector holding human NKX2-8 cDNADepositorInsertNKX2-8 (NKX2-8 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Barr2 flag
Plasmid#14685DepositorInsertbeta-arrestin 2 (Arrb2 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only