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3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pF151 pcDNA3.1(+)SOD1WT

Plasmid

#26397

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

Plasmid

#232930

Purpose

Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Flag-Halo-CTCF WT

Use

Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 VPR

Plasmid

#188511

Purpose

Expresses FLAG tagged SpRY dCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AB-GFP

Plasmid

#72203

Purpose

screen AB42 variants for aggregation

Depositor

Michael Hecht

Article

Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Insert

AB42 (APP Human)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pF146 pSOD1WTAcGFP1

Plasmid

#26407

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI3

Plasmid

#223800

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-Flag-natT1R3

Plasmid

#113950

Purpose

mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

residues 21-852

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-shRNA

Plasmid

#82697

Purpose

For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

RNAi

Promoter

hPGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 KRAB

Plasmid

#188501

Purpose

Expresses FLAG tagged SpRY dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV CAMKII PSAM4 GlyR IRES EGFP

Plasmid

#119744

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CamKII

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAV SYN PSAM4 GlyR IRES EGFP

Plasmid

#119742

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

Plasmid

#206860

Purpose

An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

Depositor

William Lagor

Article

De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

Insert

mLdlr gRNA (Ldlr Mouse)

Use

AAV and CRISPR

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKL-GFP

Plasmid

#138289

Purpose

Mammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKL (PFKL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeta-lacZ-attBx2

Plasmid

#124445

Purpose

Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Type

Empty backbone

Use

Enhancer reporter assay construct

Tags

LacZ

Expression

Mammalian

Promoter

beta globin minimal promoter

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5B-FLAG

Plasmid

#72195

Purpose

Expresses full-length Unc5b with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b (Unc5b Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only