We narrowed to 4,635 results for: abo

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pcmv3-IRG1-L272Q-HA

Plasmid

#198184

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The leucine in IRG1 at position 272 mutates to gl…

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H159Q-HA

Plasmid

#198183

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 159 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K

Plasmid

#16642

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

E545K; see note below

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H277Y-HA

Plasmid

#198185

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 277 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)

Plasmid

#214876

Purpose

Lentiviral vector encoding RfxCas13d nontargeting control guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crNT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-AIF

Plasmid

#16182

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Apoptosis-inducing factor (AIFM1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1

Plasmid

#71858

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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peGFPC2-CARKL

Plasmid

#51748

Purpose

expression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK)

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer4

Plasmid

#138576

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 S269D

Plasmid

#221319

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S269D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEX-GFP-hLC3WT

Plasmid

#24987

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Tags

N terminal Myc tag

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_PGK-Cas9-P2A-HygR

Plasmid

#164135

Purpose

Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

Plasmid

#139844

Purpose

Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK-Kinase-dead (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Mutation

K47R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHCD1E

Plasmid

#102956

Purpose

gene cloning of human CD1E

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

CD1E cDNA (CD1E Human)

Use

Cloning

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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