We narrowed to 4,803 results for: human cdnas

1. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

   Plasmid

   #63565

   Purpose

   Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

   Plasmid

   #83458

   Purpose

   Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Amino-acids 4127-4303, S4144D

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nrf1-Myc pcDNA3.1(+)-C-Myc

   Plasmid

   #169125

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-CD16.7-hPC1-eGFP-3HA

   Plasmid

   #108401

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

   Plasmid

   #209083

   Purpose

   SNCA (Avi tagged) expression along with BirA ligase

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   BirA
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

   Plasmid

   #228533

   Purpose

   A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

   Plasmid

   #168097

   Purpose

   HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nGFP-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-gp78-FLAG RING mutant C356G H361A

   Plasmid

   #61751

   Purpose

   express C-terminally FLAG tagged human gp78

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C356G H361A; P179L, F262L, Y315C, and D346G (ple…

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

   Plasmid

   #58275

   Purpose

   mammalian expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid

    #168099

    Purpose

    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nUbc-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2-Cx43-M100/125/147/213-siResist

    Plasmid

    #49858

    Purpose

    Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100, Methionine 125, Methionine 147, an…

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

    Plasmid

    #59827

    Purpose

    Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

    Plasmid

    #70667

    Purpose

    Human mutant of E2F1 lacking amino acids 193-359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 193 to 359 (E2F1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacks amino acids 193-359

    Promoter

    cmv

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

    Plasmid

    #70669

    Purpose

    Human mutant of E2F1 lacking amino acids 284 to 359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 284 to 359 (E2F1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks amin acids 284 to 359

    Promoter

    cmv

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Nrf1-Myc pcDNA3.1(+)-C-Myc

    Plasmid

    #169125

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid

    #168102

    Purpose

    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nEPEA-(EAAAK)3-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

    Plasmid

    #99746

    Purpose

    Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Made six (6) silent mutations to confer resistanc…

    Promoter

    CMV/TO

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

    Plasmid

    #164002

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1461R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

    Plasmid

    #164001

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W1460A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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