We narrowed to 2,618 results for: ubiquitin

1. pMT2 Beta1b GFP

   Plasmid

   #89893

   Purpose

   Mammalian expression of Beta1b GFP

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   GFP sequence inserted just before the Stop codon

   Promoter

   adenovirus major late promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT2 Beta1b mCherry

   Plasmid

   #89892

   Purpose

   Mammalian expression of Beta1b mCherry

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mCherry sequence inserted just before the Stop co…

   Promoter

   adenovirus major late promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZsGreen1-cMYC/pLVX-Puromycin

   Plasmid

   #180278

   Purpose

   Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

   Depositor

   Andre Catic

   Article

   Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

   Insert

   ZsGreen1-cMYC fusion protein MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-hARdelta120-472

   Plasmid

   #89088

   Purpose

   mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

   Depositor

   Elizabeth Wilson

   Article

   Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

   Insert

   hAR-120-472 N-term deletion (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-120-472

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRIPZ-CTID195-GSQ-UBnc-PURO

   Plasmid

   #208037

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   Ubnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Expression

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-Flag-Mbd3 delta1-70

   Plasmid

   #52372

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-70

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-Flag-Mbd3 delta49-63

   Plasmid

   #52374

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 49-63

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIZ GFP PODXL 4K>R

    Plasmid

    #202420

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    K431/525/526/547R

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-LucAL

    Plasmid

    #174049

    Purpose

    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucAL
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…

    Promoter

    Human Ubiquitin C

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-LucA

    Plasmid

    #174047

    Purpose

    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucA
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    ALG8 alanine 506 to threonine

    Promoter

    Human Ubiquitin C

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-LucL

    Plasmid

    #174048

    Purpose

    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucL
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    LAMA4 glycine 1254 to valine

    Promoter

    Human Ubiquitin C

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

    Plasmid

    #99746

    Purpose

    Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Made six (6) silent mutations to confer resistanc…

    Promoter

    CMV/TO

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

    Plasmid

    #99743

    Purpose

    Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Changed both L165 and E166 to Alanine

    Promoter

    CMV

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid

    #203798

    Purpose

    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQCXIZ GFP PODXL FKBP 4K>R

    Plasmid

    #202421

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    FKBP and GFP

    Expression

    Mutation

    K431/525/526/547R

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid

    #203797

    Purpose

    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid

    #86455

    Purpose

    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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