Skip to main content
Addgene
Showing: 481 - 520 of 621 results

  6. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Promoter
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

    Enlarge

  11. pMuLE Lenti Dest Neo

    Plasmid
    #62178
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62178-plasmid-map-sequence-id-101462

    Enlarge

  12. pmirGLO-Bmpr2-1719

    Plasmid
    #49379
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49379-plasmid-map-sequence-id-73402

    Enlarge

  14. pmirGLO-Bmpr2-Δ17

    Plasmid
    #49380
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    changed nucleotides AC to TG at bp 7347-7348
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49380-plasmid-map-sequence-id-73403

    Enlarge

  15. pmirGLO-Bmpr2-Δ19

    Plasmid
    #49381
    Purpose
    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-19 binding site. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Bmpr2 (BMPR2 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    changed nucleotides TG to AC at bp 7344-7345
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49381-plasmid-map-sequence-id-73404

    Enlarge

  21. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid
    #51006
    Purpose
    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP
    Use
    Lentiviral
    Tags
    histone 2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51006-plasmid-map-sequence-id-77912

    Enlarge

  24. cEF1a-GFP

    Plasmid
    #135671
    Purpose
    Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    135671-plasmid-map-sequence-id-325845

    Enlarge

  25. cSFFV-GFP

    Plasmid
    #135670
    Purpose
    Introduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    135670-plasmid-map-sequence-id-325843

    Enlarge

  26. cTRE-GFP

    Plasmid
    #135665
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135665-plasmid-map-sequence-id-325434

    Enlarge

  32. pIALD2HMGr

    Plasmid
    #185867
    Purpose
    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185867-plasmid-map-sequence-id-370811

    Enlarge

  33. cEF1a-LSL-GFP

    Plasmid
    #135672
    Purpose
    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135672-plasmid-map-sequence-id-325851

    Enlarge

  34. pDUAL OCLN (GFP)

    Plasmid
    #86982
    Purpose
    Lentiviral expression construct encoding Occludin and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    OCLN (OCLN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86982-plasmid-map-sequence-id-173293

    Enlarge

  35. cTRE-GFP-miR30_shRen

    Plasmid
    #135666
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shRenilla into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135666-plasmid-map-sequence-id-325436

    Enlarge

  38. miR-144-451 Trap

    Plasmid
    #61488
    Purpose
    to knockdown endogenous miR144 and miR451 levels
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    miR-144-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. cTRE-GFP-miR30_shPten.1523

    Plasmid
    #135667
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135667-plasmid-map-sequence-id-325441

    Enlarge

Showing: 481 - 520 of 621 results