We narrowed to 11,597 results for: 110

1. pLenti-TRE3G-BE3RA-PGK-Puro

   Plasmid

   #110846

   Purpose

   Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   TRE3G

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_CACNB1_Guanylate-kin

   Plasmid

   #110036

   Purpose

   Protein expression and purification of CACNB1_Guanylate-kin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CACNB1_Guanylate-kin (CACNB1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL410-BRN2p

   Plasmid

   #110733

   Purpose

   Luciferase reporter for the human BRN2 promoter

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   BRN2 promoter luciferase (POU3F2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. hOC-GFPtpz

   Plasmid

   #110207

   Purpose

   Expresses GFP in mature bone cells.

   Depositor

   David Rowe

   Article

   Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

   Insert

   eGFPtopaz
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Human osteocalcin (BGLAP)

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF-T20S

   Plasmid

   #110805

   Purpose

   expresses Thermoplasma acidophilium 20S proteasome in E. Coli

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Inserts

   PsmB
   PsmA
   

   Use

   Tags

   tev-His

   Expression

   Bacterial

   Mutation

   N2D and Q2E

   Promoter

   T7 and T7 promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-FNLS-PGK-Puro

   Plasmid

   #110844

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-Citrine2

    Plasmid

    #110772

    Purpose

    Yellow fluorescent protein with improved photostability

    Depositor

    Robert Campbell

    Article

    Wiens et al Integr Biol (Camb). 2018 Jul 16;10(7):41

    Insert

    Citrine2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV/myc/ER-αT9ib

    Plasmid

    #110768

    Purpose

    Expresses ER intrabody against mouse and human TLR9

    Depositor

    Thomas Böldicke

    Article

    Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

    Insert

    intrabody against mouse and human TLR9
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK569

    Plasmid

    #110614

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V32M (see comments below)

    Promoter

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLAG-ZYG11A

    Plasmid

    #110551

    Purpose

    expresses a FLAG-tagged human ZYG11A protein in mamalian cells

    Depositor

    Edward Kipreos

    Article

    Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

    Insert

    ZYG11A (ZYG11A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Use

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBTK501

    Plasmid

    #110602

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXNPH3

    Plasmid

    #110098

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-PKCZ

    Plasmid

    #110512

    Purpose

    Fluorescent PKCZ

    Depositor

    Bernardo Mainou

    Insert

    PKCZ (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBTK229

    Plasmid

    #110591

    Purpose

    BTK Type 3 coding sequence for golden gate assembly and homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Kanamycin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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