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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,930 results for: 158

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Showing: 241 - 260 of 10930 results

  1. pNHEJ-Cas12a

    Plasmid
    #158713
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158713-plasmid-map-sequence-id-320845

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  3. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  4. p663-UBC-V5-TurboID-V5-STOP_IDG-K

    Plasmid
    #158159
    Purpose
    Gateway destination clone of TurboID-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158159-plasmid-map-sequence-id-309970

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  5. pLKO_HA.V5.VSVg_NGFR

    Plasmid
    #158244
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158244-plasmid-map-sequence-id-327705

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  19. pHR SFFV GLP1R(S301A)-EGFP

    Plasmid
    #158123
    Purpose
    Lentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.
    Depositor
    Neal Devaraj
    Insert
    GLP1R (GLP1R )
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Serine 301 changed to alanine to enhance membrane…
    Promoter
    SFFV
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    158123-plasmid-map-sequence-id-309384

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Showing: 241 - 260 of 10930 results