We narrowed to 858 results for: PX330

1. px330 EZH2 - pDY052

   Plasmid

   #171107

   Purpose

   Expresses Cas9 and an sgRNA targeting EZH2

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. beta2 (px330:2-guideCas9)

   Plasmid

   #169435

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-UFM1 sgRNA2

   Plasmid

   #134634

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA2 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330-UFM1 sgRNA1

   Plasmid

   #134633

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA1 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Puro-ccdB

   Plasmid

   #82580

   Purpose

   A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

   Depositor

   Ivo Huijbers

   Article

   Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GluA3 (px330:2-guideCas9)

   Plasmid

   #169424

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP926-pX330-Batf3

   Plasmid

   #181772

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Batf3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330 NIPBL gRNA

    Plasmid

    #165590

    Purpose

    Insertion of NIPBL degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    NIPBL (Nipbl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-GFP-SFPQ

    Plasmid

    #97084

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA GFP-SFPQ
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid

    #172828

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330 RAD21 gRNA

    Plasmid

    #165592

    Purpose

    Insertion of RAD21 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-PITCh-TUBB4B

    Plasmid

    #207785

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 p300 gRNA

    Plasmid

    #165591

    Purpose

    Insertion of p300 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    P300 (Smarca2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge