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pRS413 Prp5 WT

Plasmid

#87599

Purpose

Encodes Prp5 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 )

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xDHFR

Plasmid

#20218

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Rp51a Hyperstabilized 5'SS

Plasmid

#100988

Purpose

pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut ATG/GTATGT -> AAG/GTAAGT

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-NoBS-NoPseudoBS

Plasmid

#100986

Purpose

RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLNC Int3HA

Plasmid

#21329

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Int3 (Notch4 Mouse)

Use

Retroviral

Tags

HA

Available Since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-HA-TEV

Plasmid

#105769

Purpose

Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

HA-TEV

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-HA

Plasmid

#105785

Purpose

Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-FLAG

Plasmid

#105783

Purpose

Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHA2

Plasmid

#85710

Purpose

This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Tags

VP16 AD (413-490)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

Plasmid

#62930

Purpose

pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.1Synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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NUMB4-eGFP-M107K

Plasmid

#37805

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 4 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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pJEP217-Lenti-CMV-GFP-WPRE-pA

Plasmid

#62925

Purpose

pLenti vector backbone designed to express GFP from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.3αCaMKII and CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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env8 reverse J1/13

Plasmid

#99831

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 reverse J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 sequence is reversed

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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