Addgene

We narrowed to 1,466 results for: calcium

Showing: 241 - 260 of 1466 results

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pCDNA3.1(+)-NEMOs

Plasmid

#189931

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOs

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOf

Plasmid

#189932

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOb

Plasmid

#189934

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOb

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pRSETB

Plasmid

#48203

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Use

Tags

nuclear export signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Use

Tags

nuclear export signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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N1-Tq-Ca-FLITS

Plasmid

#191454

Purpose

Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Tq-Ca-FLITS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1b

Plasmid

#63136

Purpose

Red fluorescent calcium sensor protein

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1b

Use

Tags

nuclear export signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle CMV RGECO-TnT

Plasmid

#124643

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

Depositor

Paul Robinson, Matthew Daniels, Hugh Watkins

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnT (TNNT2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2G

Plasmid

#159605

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Has Service

AAV1, AAV2, and AAV9

Insert

NIR-GECO2G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA pCAGGS

Plasmid

#206107

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

tag-168

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI W391F V398L

Plasmid

#60787

Purpose

Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Eric Schreiter, Loren Looger

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI W391F V398L

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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