Addgene

We narrowed to 6,093 results for: cat.2

Showing: 241 - 260 of 6093 results

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WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise-CAL-2-Lan-2

Plasmid

#58164

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

CAL2-Lan2 (Lan2 = mNeonGreen) and mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_eGCaMP(2+)

Plasmid

#201148

Purpose

expresses eGCaMP2+ in mammalian cells

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP2+

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-2

Plasmid

#202092

Purpose

Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
TFAP4_BHLH_3-2

Plasmid

#185055

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_3_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-2

Plasmid

#185056

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPK1E-2

Plasmid

#48146

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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shTBX5 # 2

Plasmid

#42550

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shYES1 # 2

Plasmid

#42547

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shBIRC5 # 2

Plasmid

#42554

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shYAP1 # 2

Plasmid

#42541

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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shBCL2L1 # 2

Plasmid

#42552

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBCL2L1 (BCL2L1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#2

Plasmid

#75254

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Tags

Expression

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

Plasmid

#117006

Purpose

Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Mode #2 Library (14-3-3σ)

Pooled Library

#111706

Purpose

Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only