We narrowed to 6,951 results for: crispr cas9 plasmids

1. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

   Plasmid

   #148852

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA2

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

   Plasmid

   #148853

   Purpose

   Mammalian Expression of HsTNRC6B-sgRNA3

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6B-sgRNA3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

   Plasmid

   #148857

   Purpose

   Mammalian Expression of HsPatL1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsPatL1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

   Plasmid

   #148858

   Purpose

   Mammalian Expression of HsTNRc6C-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRc6C-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

   Plasmid

   #140082

   Purpose

   Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

   Depositor

   Keith Joung

   Insert

   pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. BlatCas9

   Plasmid

   #163792

   Purpose

   The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hPLD3-sgRNA-Cas9_mcherry

   Plasmid

   #199342

   Purpose

   encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hPLD4-sgRNA-Cas9_mcherry

   Plasmid

   #199343

   Purpose

   encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   sgRNA: accagtagtatgaagccacg

   Promoter

   human U6

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NAX_CAGGS_CAS9_Blasto

    Plasmid

    #167856

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_Hygro

    Plasmid

    #167859

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NAX_CAGGS_CAS9_EBFP2

    Plasmid

    #167857

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_EYFP

    Plasmid

    #167858

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_iRFP713

    Plasmid

    #167860

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid

    #214127

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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