We narrowed to 5,561 results for: donor

1. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTopo_Ex2Donor_pPGK-PURO

   Plasmid

   #198862

   Purpose

   Donor template for CRISPR/Cas9 targeting of TERT Ex2

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   PURO
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. DUX4 pDonor221

   Plasmid

   #187801

   Purpose

   DUX4 entry clone

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   DUX4 (DUX4 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GA Donor_hEF1a

   Plasmid

   #229779

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA Donor_CAG

   Plasmid

   #229780

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_Tn-pOUT

   Plasmid

   #213905

   Purpose

   Mariner transposon with outward-facing pJ23104 promoter

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and outward-facine pJ23104 promoter
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Actb HR donor

    Plasmid

    #97317

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_T804N_hPGK1_mScarlet-I_Donor

    Plasmid

    #173207

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N mScarlet-I donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hPGK1

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonorGFP(Ptr)

    Plasmid

    #175578

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    Mini-Tn, GFP cassette
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRainbow-DONOR1

    Plasmid

    #75398

    Purpose

    CRISPRainbow-DONOR1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Cm(R)-CcdB
    

    Use

    CRISPR

    Tags

    no

    Promoter

    Bacteria

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LMNA_mScarlet-I_Donor

    Plasmid

    #178092

    Purpose

    HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA-mScarlet-I HDR donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDonorCm(Ptr)

    Plasmid

    #175577

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    Mini-Tn, Cm cassette
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDonor_Tn[sfGFP]

    Plasmid

    #213902

    Purpose

    Mariner transposon expressing sfGFP with kan selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and superfolder GFP
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDonor-LoxP

    Plasmid

    #219436

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    two transposon ends, LoxP-terminator-LoxP gene cassette
    

    Expression

    Bacterial

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL5200 (pDonor_ISGst3)

    Plasmid

    #210875

    Purpose

    Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    ISGst3
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97309

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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