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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,439 results for: nes

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Showing: 241 - 260 of 23439 results

  1. pSLQ10625

    Plasmid
    #239268
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    CAG
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239268-plasmid-map-sequence-id-474742

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  3. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. mCherry-Kinesin11-N-18

    Plasmid
    #55067
    Purpose
    Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Kinesin11 (KIF11 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55067-plasmid-map-sequence-id-244069

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  11. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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  12. mEmerald-Nesprin3-C-18

    Plasmid
    #54203
    Purpose
    Localization: Nuclear Membrane , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54203-plasmid-map-sequence-id-247990

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  13. mCherry-Farnesyl-5

    Plasmid
    #55045
    Purpose
    Localization: Plasma Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55045-plasmid-map-sequence-id-314088

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  14. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Article
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119164-plasmid-map-sequence-id-232464

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  15. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  20. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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Showing: 241 - 260 of 23439 results