We narrowed to 2,206 results for: pEGFP-c1

1. Venus-30aa-Noxa-pEGFP-C1

   Plasmid

   #166762

   Purpose

   To increase the length of the linker between Venus and Noxa proteins

   Depositor

   David Andrews

   Insert

   Noxa (PMAIP1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Venus-7aa-Cb5-pEGFP-C1

   Plasmid

   #166764

   Purpose

   To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5a Rat)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-C1-MKLP1 S708/710A

   Plasmid

   #70153

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S708A, S710A

   Promoter

   CMV

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-Clavesin2 (pEGFP-C1-Sec14b)

   Plasmid

   #47420

   Depositor

   Peter McPherson

   Article

   Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

   Insert

   Clavesin2 (CLVS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-C1 Slp2-a E11A/R32A

   Plasmid

   #40055

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp2-a E11A/R32A (Sytl2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glutamate 11 to Alanine, Arginine 32 to Alanine

   Promoter

   CMV promoter

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-C1-HsTNRC6C_1208-1690-CIM1CIM2mut_U

   Plasmid

   #147678

   Purpose

   Mammalian Expression of HsTNRC6C_1208-1690-CIM1CIM2mut

   Depositor

   Elisa Izaurralde

   Article

   Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

   Insert

   HsTNRC6C_1208-1690-CIM1CIM2mut (TNRC6C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C1-HsTNRC6C_1208-1690-M2WA_U

   Plasmid

   #147679

   Purpose

   Mammalian Expression of HsTNRC6C_1208-1690-M2WA

   Depositor

   Elisa Izaurralde

   Article

   Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

   Insert

   HsTNRC6C_1208-1690-M2WA (TNRC6C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Venus-Bik-cb5-pEGFP-C1

   Plasmid

   #177431

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Bik MBR removed (after position 136) and replaced…

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus-Bik-delMBR-pEGFP-C1

   Plasmid

   #177432

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Bik MBR removed (after position 136)

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-7aa-cb5-pEGFP-C1

    Plasmid

    #177407

    Purpose

    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Venus-BclXL-ActA-pEGFP-C1

    Plasmid

    #177410

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-ActA, BclX-acta
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid

    #177412

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-ActA, BclX-acta
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid

    #177414

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-Cb5, BclX-cb5
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Venus-BclXL-Cb5-pEGFP-C1

    Plasmid

    #177415

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-XL-Cb5, BclX-cb5
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCerulean3-Bcl2-ActA-pEGFP-C1

    Plasmid

    #177418

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid

    #177419

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCerulean3-Bcl2-cb5-pEGFP-C1

    Plasmid

    #177421

    Purpose

    Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid

    #177422

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

    Depositor

    David Andrews

    Insert

    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Venus-Bik-L61G-pEGFP-C1

    Plasmid

    #177426

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Leucine 61 replaced with glycine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge