We narrowed to 10,582 results for: plasmids 101

1. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Hygro-iRFP670

   Plasmid

   #99377

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-iRFP670
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

   Plasmid

   #204151

   Purpose

   Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   SARS-CoV-2 Delta variant spike
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   TRE3G

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-pur-CAG-Bi-DREADD

   Plasmid

   #159457

   Purpose

   AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   Bi-DREADD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fusion protein of hM3Dq-mCherry and HA-KORD seper…

   Promoter

   CAG

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

   Plasmid

   #209198

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Article

   Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOGS539_GLuc-ns

    Plasmid

    #226719

    Purpose

    Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE3_ mTFP1- Vamp2

    Plasmid

    #201214

    Purpose

    AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Insert

    mTFP1 (from addgene #54613)
    

    Use

    AAV

    Tags

    mouse Vamp2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

    Plasmid

    #166018

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Tnnt2 -Atp2a2-HA

    Plasmid

    #227804

    Purpose

    cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of mouse Atp2a2 and a HA tag

    Depositor

    Yuxuan Guo

    Article

    Chen et al Fundam Res. 2025 Jun 30. doi: https://do

    Insert

    Atp2a2-HA (Atp2a2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tnnt2

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ENTR_BN001T-N_START

    Plasmid

    #218058

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN001T-N_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ENTR_BN006A-P_START

    Plasmid

    #218059

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN006A-P_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ENTR_BN001M-T_START

    Plasmid

    #218060

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN001M-T_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AF10_OM-LZ

    Plasmid

    #111753

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    MLLT10 (MLLT10 Human)
    

    Use

    Tags

    6x His - TEV

    Expression

    Bacterial

    Mutation

    Contains the OM-LZ region of AF10 (residues 720-7…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW1221

    Plasmid

    #229873

    Purpose

    gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    QF-act-4 3' UTR::SEC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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