We narrowed to 16,615 results for: puromycin

1. pLenti-puro

   Plasmid

   #39481

   Purpose

   Tetracycline inducible 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5 and 6XHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-CMV-Flag-CDK12_IDG-K

   Plasmid

   #135281

   Purpose

   Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-CDK12 (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Sec31a #861#640

   Plasmid

   #42123

   Depositor

   Ayano Satoh

   Article

   Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

   Insert

   Sec31a (SEC31A Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   Tet ON

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TO-hNGN2-BSD-mApple

   Plasmid

   #182308

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2 (NEUROG2 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLC-ZsGreen-P2A-Puro

   Plasmid

   #124302

   Purpose

   Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   ZsGreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-mCherry-Mango II x 24

   Plasmid

   #127587

   Purpose

   Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

   Depositor

   David Rueda

   Article

   Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

   Insert

   mCherry-Mango II x24
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   pTRE Tet ON

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-FR-GECO1a

   Plasmid

   #163681

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1a

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1a
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM705 Gsq

    Plasmid

    #109356

    Purpose

    GsX chimera with Gαq C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsq chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM705 Gs13

    Plasmid

    #109359

    Purpose

    GsX chimera with Gα13 C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gs13 chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM705 Gs12

    Plasmid

    #109357

    Purpose

    GsX chimera with Gα12 C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gs12 chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVpuro-CMV-N-EGFP

    Plasmid

    #122848

    Purpose

    N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 GFP shRNA

    Plasmid

    #30323

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    GFP shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-BC-EF1a-Puro

    Plasmid

    #127170

    Purpose

    PCR template for EF1a-puro resistance cassette

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJC5-3XHA-EGFP-PEX26

    Plasmid

    #139054

    Purpose

    HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XHA-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    UBC

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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