We narrowed to 31,194 results for: uros

1. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER retro puro macroH2A2 shRNA

   Plasmid

   #30518

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6. ():.

   Insert

   mH2A2 shRNA
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. H446 EF1a RA-ABE6.3 p2A Puro

   Plasmid

   #139104

   Purpose

   Lentiviral expression of ABE6.3.

   Depositor

   Wen Xue

   Article

   Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

   Insert

   ABE RA6.3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ef1a-somBiPOLES-mCerulean-IRES-Puro

   Plasmid

   #192585

   Purpose

   lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   Lentiviral

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYJ21-lenti-EF1a-ASAP2f-puro

   Plasmid

   #131065

   Purpose

   Forced expression of ASAP2f

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

   Insert

   ASAP2f
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-Tet-puro-hARAF-shRNA-1

   Plasmid

   #185370

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF

   Depositor

   Ayaz Najafov

   Insert

   ARAF (ARAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-2xFLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172628

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV UAS-DsRed-Express2-PGK-Puro

   Plasmid

   #190801

   Purpose

   LV construct encoding UAS-DsRed-Express2 reporter gene.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   UAS-T2A-DsRed-Express2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-U6-sgRNA-PGK-puromycin-AflII

   Plasmid

   #106404

   Purpose

   Empty guide RNA cloning vector with an AflII site added from Addgene 41824

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

    Plasmid

    #189955

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_MMERVK10c
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid

    #161587

    Purpose

    expression of Myc-tagged mouse Lrrk2 without GTPase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1023-1879

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid

    #161586

    Purpose

    expression of Myc-tagged mouse Lrrk2 without kinase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1871-2540

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p7270 MSCV-N-HA-IRES-PURO Empty

    Plasmid

    #163302

    Purpose

    Empty vector control

    Depositor

    Peter Howley, Elizabeth White

    Article

    White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid

    #157981

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid

    #172298

    Purpose

    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-PuroR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1120 pBabe puroL HA FKHR H215R

    Plasmid

    #9026

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pKI-cjFOXL2-coemiRFP670-PGK-Puro

    Plasmid

    #186168

    Purpose

    Knock-in gene targeting in marmoset cells at FOXL2 locus

    Depositor

    Sho Yoshimatsu

    Article

    Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

    Insert

    emiRFP670
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe puro H-Ras 12V (37G)

    Plasmid

    #12589

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    HRas 12V 37G (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V, 37G

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only