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pFloxin-IRES-Ofd1-Myc-S75F

Plasmid

#24574

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc-A80T

Plasmid

#24575

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc-G139S

Plasmid

#24576

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Glycine 139 changed to serine (codon GGT to AGT)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-Ofd1-Myc-S437R

Plasmid

#24577

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-DR RecR(K23A/R27A)

Plasmid

#12619

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Mutation

changed Lysine 23 to Alanine, and Arginine 27 to …

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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mCardinal-N1

Plasmid

#54590

Purpose

Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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IDP425

Plasmid

#87022

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 Smad1

Plasmid

#14045

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

Plasmid

#69908

Purpose

Expresses a GFP circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-O-GECO1

Plasmid

#46025

Purpose

Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

O-GECO1

Expression

Mammalian

Mutation

R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pENO1-iRFP-NATr

Plasmid

#129731

Purpose

Integration plasmid to overexpress iRFP in Candida albicans

Depositor

Robert Wheeler

Article

Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

Insert

iRFP-Candida albicans codon-optimized

Expression

Yeast

Promoter

ENO1 from Candida albicans

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pME-nlsGFP-P2A

Plasmid

#80808

Purpose

nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAYP (pDre)

Plasmid

#62709

Purpose

Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

Dre

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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