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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 2641 - 2660 of 12310 results

  4. pcDNA3-AktAR2-NLS

    Plasmid
    #175012
    Purpose
    Nuclear-targeted AktAR2.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AktAR-NLS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175012-plasmid-map-sequence-id-343414

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  8. pNOC-CRISPR-GFP

    Plasmid
    #100009
    Purpose
    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome
    Depositor
    Eva Farre
    Publication
    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968
    Type
    Empty backbone
    Use
    Algae, nannochloropsis expression
    Tags
    Cas9-GFP
    Promoter
    Ribi promoter
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    100009-plasmid-map-sequence-id-202006

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  11. pcDNA3-Akt-STOPS-NES

    Plasmid
    #175008
    Purpose
    Cytosol-targeted Akt-STOPS.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175008-plasmid-map-sequence-id-344936

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  13. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  15. pEGFP-NUP188

    Plasmid
    #159893
    Purpose
    Expresses NUP188 in mammalian cells
    Depositor
    Kozo Tanaka
    Publication
    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NUP188 (NUP188 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    To avoid NotI site, first three amino acids are d…
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159893-plasmid-map-sequence-id-313971

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  17. pCMV-hARdelta538-614

    Plasmid
    #89107
    Purpose
    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain
    Depositor
    Elizabeth Wilson
    Publication
    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAR-538-614 (AR Human)
    Expression
    Mammalian
    Mutation
    hAR delta 538-614
    Promoter
    CMV
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. pCAG-spCas9-SB

    Plasmid
    #169919
    Purpose
    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SB100X
    Use
    CRISPR
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169919-plasmid-map-sequence-id-336025

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  20. pCS2+ GCamp6-3NLS

    Plasmid
    #165401
    Purpose
    Expresses a nuclear localized genetically encoded calcium reporter.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Localization Signal
    Use
    Avian, xenopus, zebrafish
    Tags
    GCamp6
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165401-plasmid-map-sequence-id-325983

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Showing: 2641 - 2660 of 12310 results