We narrowed to 10,124 results for: Met

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pcDNA3.1/NDUFS7/myc-His

Plasmid

#127488

Purpose

Expresses myc-tagged NDUFS7 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Publication

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS7 (NDUFS7 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3C-BE3

Plasmid

#113412

Purpose

Expresses hA3C-BE3 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_CR_K95M

Plasmid

#108106

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation),…

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ΔD-box-CycA-GFP (525)

Plasmid

#39856

Depositor

Jonathon Pines

Publication

den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Insert

Cyclin A2 (CCNA2 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

L2V; Δ destruction box = deletion of aa47-55

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Epac-S-H74

Plasmid

#170338

Purpose

mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Publication

Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

Insert

mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Publication

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus2-WTSTAT3

Plasmid

#123165

Purpose

Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Publication

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

none

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST-hMeCP2-GFP-G273X

Plasmid

#48080

Purpose

Mammalian expression of GFP tagged MeCP2-G273X

Depositor

Huda Zoghbi

Publication

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

expresses AT-Hook G273X

Promoter

CMV

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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GST-SmB

Plasmid

#1841

Depositor

Philip Leder

Publication

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB (SNRPB Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pQFKan

Plasmid

#32321

Depositor

Steve Stowers

Publication

Stowers et al Fly (Austin). 2011 Oct 1;5(4).

Insert

QF Kan

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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FRP1671

Plasmid

#64538

Purpose

Inducible MET15 gene

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

MET15 (MET17 Budding Yeast)

Expression

Yeast

Promoter

(lexA-box)_4-P_CYC1_minimal

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-RKIP_RFP

Plasmid

#199215

Purpose

RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.

Depositor

Gabor Balazsi

Publication

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

RKIP (PEBP1 Human)
RFP

Expression

Mammalian

Promoter

CMV and PGK

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Publication

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neoBeta2mcherry

Plasmid

#45097

Depositor

Henry Lester

Publication

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 mcherry (Chrnb2 Mouse)

Tags

has c-myc tag

Expression

Mammalian

Mutation

β2 was cloned into pCI-neo at the restriction sit…

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRQ-hRasV12

Plasmid

#18113

Depositor

Alex Swarbrick

Publication

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

h-RasV12G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V, constitutively active

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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MT1E

Plasmid

#105702

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1e

Depositor

Artur Krężel

Publication

Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

Insert

Metallothionein 1e (MT1E Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Publication

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pALS3-Ma-sfGFP-WT

Plasmid

#212119

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

Depositor

Ryan Mehl

Inserts

SUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)

Tags

His6

Expression

Bacterial

Promoter

araC and lpp

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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