We narrowed to 5,851 results for: KRAS

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pLenti-CMV-cGAS-N-HA (murine)

Plasmid

#130916

Purpose

Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

murine cGAS N terminus

Tags

HA

Expression

Mammalian

Mutation

murine cGAS N terminus (aa 1-147)

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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p221- purVSG117UTR

Plasmid

#59732

Purpose

Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

Depositor

Gloria Rudenko

Article

Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

Insert

VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site

Use

For homologous recombination into vsg221 expressi…

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Sintbad

Plasmid

#234668

Purpose

expresses SINTBAD (TBKBP1) in mammalian cells

Depositor

Lienhard Schmitz

Article

Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

Insert

SINTBAD (TBKBP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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SpRYc

Plasmid

#175575

Purpose

Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

Insert

SpRYc

Tags

EGFP

Expression

Mammalian

Mutation

PID of SpRY grafted onto the N-terminal domain of…

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTCas9_scr

Plasmid

#207566

Purpose

ThermoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-JUN

Plasmid

#79512

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

JUN (JUN Human)

Use

Gateway Cloning

Tags

HA Tag

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-FOS

Plasmid

#79511

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

FOS (FOS Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-SRRM2-gRNA

Plasmid

#238251

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SRRM2

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-SRRM2-repair-template

Plasmid

#238247

Purpose

For knock-in msfGFP to SRRM2 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SRRM2

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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AA364

Plasmid

#215956

Purpose

Fragmid fragment: (guide cassette) epegRNA expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLgw RFC1-V5-EcoDam

Plasmid

#59205

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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B-HiFi SpCas9

Plasmid

#207362

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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DRH335: FCK-Optopatch1

Plasmid

#51695

Purpose

Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch1

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20-PAAA

Plasmid

#218581

Purpose

Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed Proline 172 to Alanine and Tyrosine 174 t…

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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