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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Publication

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV-H2B-mScarlet-I

Plasmid

#182297

Purpose

Localization of mScarlet-I fluorescent protein to histone 2B

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Tags

mScarlet-I

Expression

Mammalian

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAd-Track HA PGC-1 alpha

Plasmid

#14427

Depositor

Pere Puigserver

Publication

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSPble

Plasmid

#63561

Purpose

Bleomycin drug selection marker

Depositor

Greg Matlashewski

Publication

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Bleomycin drug selection marker

Use

Leishmania expression

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUb6-Zpo1-V5/His

Plasmid

#29323

Depositor

Zena Werb

Publication

Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

Insert

Zeppo1

Tags

His and V5

Expression

Mammalian

Available since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Tags

C-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LeGO-iV

Plasmid

#27360

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, IRES-Venus

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SENP6 (catalytic domain)

Plasmid

#16359

Depositor

Guy Salvesen

Publication

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP6 catalytic domain (SENP6 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 628 to 1112 catalytic domain of human …

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUD-MSP1D1

Plasmid

#161608

Purpose

Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Publication

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

UD-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans and tyrosines w…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-28c(+)-YTHDF2

Plasmid

#102275

Purpose

For bacterial expression of His-tagged human YTHDF2

Depositor

Samie Jaffrey

Publication

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF2 (YTHDF2 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFLEX+mCyclin D1

Plasmid

#86793

Purpose

mammalian expression of cyclin D1

Depositor

Martine Roussel, Charles Sherr

Publication

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cyclin D1 (Ccnd1 Mouse)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Publication

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p3990 pET ubcH8

Plasmid

#8650

Depositor

Peter Howley

Publication

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

UbcH8 (UBE2L6 Human)

Tags

His

Expression

Bacterial

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pBabe hygro myc p110-CAAX

Plasmid

#12591

Depositor

Channing Der

Publication

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

p110-CAAX (PIK3CA Bovine)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBa-FRB-3myc-KIF1A tail 391-1698

Plasmid

#64286

Purpose

KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Publication

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF1A (Kif1a, Kif1a Rat, Mouse)

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa 391-1698, 5'end mostly matches rat, 3…

Promoter

chicken Beta Actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Publication

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSA1

Plasmid

#52261

Purpose

Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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