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We narrowed to 17,951 results for: puro

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Showing: 2661 - 2680 of 17951 results

  2. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171801-plasmid-map-sequence-id-337972

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  4. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  6. integrin beta6 Y774F pBabe puro

    Plasmid
    #13599
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 Y774F (ITGB6 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Y774F
    Available Since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  10. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    Ef1-alpha
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  12. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  13. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  14. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid
    #161587
    Purpose
    expression of Myc-tagged mouse Lrrk2 without GTPase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1023-1879
    Promoter
    CMV
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161587-plasmid-map-sequence-id-318592

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  15. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid
    #161586
    Purpose
    expression of Myc-tagged mouse Lrrk2 without kinase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1871-2540
    Promoter
    CMV
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161586-plasmid-map-sequence-id-318591

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  19. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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Showing: 2661 - 2680 of 17951 results