We narrowed to 152,655 results for: des

1. plenti hSyn AldoA

   Plasmid

   #220916

   Purpose

   Neuronal specific expression of AldoA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   AldoA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCSF107mT-GATEWAY-3'-3HA

   Plasmid

   #67616

   Purpose

   A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   three inframe 3' HA tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pENTR223-BFRF1

   Plasmid

   #195801

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BFRF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BFRF1 (BFRF1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR223-EBNALP

   Plasmid

   #195817

   Purpose

   Gateway Entry Clone of Epstein-Barr virus EBNALP

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   EBNALP (EBNA-LP Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Luciferase shRNA

   Plasmid

   #225343

   Purpose

   shRNAmir backbone for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   Luciferase shRNA (LOC116160065 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAICS-2XStrep

   Plasmid

   #125683

   Purpose

   expresses human PAICS protein with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PAICS (PAICS Human)
   

   Use

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMW35 p21 (mouse)

   Plasmid

   #16478

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 (Cdkn1a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pZVsE_SC12

   Plasmid

   #219722

   Purpose

   Zika virus stable E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   ZKV sE SC12
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A264W, T267R, S286W, S287P, G29K, A25M

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pENTR223-BZLF2

    Plasmid

    #195784

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BZLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BZLF2 (BZLF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-LMP2A-IRES-GFP

    Plasmid

    #220352

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV-EF1aGFP-W

    Plasmid

    #159291

    Purpose

    Lentiviral vector expressing GFP driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 Ub2-UBB+1

    Plasmid

    #23968

    Depositor

    Nico Dantuma

    Article

    Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

    Insert

    Ubiquitin B +1 transcript (UBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD2sE_SC12

    Plasmid

    #219719

    Purpose

    DV2 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV2 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs_FLAG-GGC1

    Plasmid

    #133258

    Purpose

    Express FLAG-tagged GGC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    GGC1 (GGC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Human SHIP1 (clone hp51CN)

    Plasmid

    #123347

    Purpose

    Expression of human SHIP1

    Depositor

    Christophe Erneux

    Article

    Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

    Insert

    INPP5D (INPP5D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-LMP1-IRES-GFP

    Plasmid

    #220350

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PLKO-mcherry-luc-puro-shSCR

    Plasmid

    #29780

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    shSCR
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pENTR-GFI1

    Plasmid

    #16168

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Growth factor independent 1 (GFI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

24. RHBDD1 pEGFP-N2 (1078)

    Plasmid

    #62028

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRIP-SFFV-GFP-RELA K310R

    Plasmid

    #196630

    Purpose

    Express GFP-RELA K310R

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    GFP-RELA K310R (RELA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    K310R

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pWPI-GFP-3xFLAG

    Plasmid

    #201639

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs_FLAG-NDT1

    Plasmid

    #133254

    Purpose

    Express FLAG-tagged NDT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 (YIA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmcherry α-catenin M319G/R326E

    Plasmid

    #178648

    Purpose

    express α-catenin with M319G/R326E mutation

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    M319G/R326E

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-ABE-NT-sgRNA

    Plasmid

    #112734

    Purpose

    AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE-NT-sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR223-BXLF2

    Plasmid

    #195830

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BXLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BXLF2 (BXLF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR223-BMRF2

    Plasmid

    #195803

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BMRF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BMRF2 (BMRF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR223-BKRF2

    Plasmid

    #195773

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BKRF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BKRF2 (BKRF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs_FLAG-MCART6

    Plasmid

    #133251

    Purpose

    Express FLAG-tagged MCART6 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART6 (SLC25A53 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid

    #65263

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CDK4-luciferase full length (2135)

    Plasmid

    #86656

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    2120 base pairs including up to coding nucleotide…

    Promoter

    CDK4

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMXs_FLAG-MCART2

    Plasmid

    #133250

    Purpose

    Express FLAG-tagged MCART2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART2 (SLC25A52 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge