We narrowed to 10,077 results for: ATC

1. pAT15516_BEAR-GFP-2in1

   Plasmid

   #174082

   Purpose

   BEAR-GFP plasmid with a gRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TRE3G-MYCN

   Plasmid

   #104542

   Purpose

   PiggyBac vector encoding doxycycline-inducible human MYCN

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIRECT_23C

   Plasmid

   #91140

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF112

   Plasmid

   #135920

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Luciferase; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyDock

   Plasmid

   #124618

   Purpose

   Expresses SpyDock to bind SpyTag non-covalently for affinity purification

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyDock
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-AviTag-DogTag-MBP

   Plasmid

   #171773

   Purpose

   Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogTag-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIRECT_21C

   Plasmid

   #91130

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPRv2 NT sgRNA3

   Plasmid

   #164929

   Purpose

   Expresses Non-targeting sgRNA3 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA3 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF123

   Plasmid

   #135921

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMD19_STARR-seq_plants

    Plasmid

    #117379

    Purpose

    STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

    Depositor

    Xiaoyu Zhang

    Article

    Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    GFP derived from pMDC107

    Expression

    Plant

    Mutation

    Promoter

    35s core promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT9651-BEAR-GFP

    Plasmid

    #162989

    Purpose

    BEAR target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FLEX-NBL10

    Plasmid

    #69971

    Purpose

    Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

    Depositor

    Jeffrey Friedman

    Article

    Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

    Insert

    NBL10
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid

    #88879

    Purpose

    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP fusion protein and HA tag

    Expression

    Mammalian

    Mutation

    amino acids 4-460 only; L78P & L287P *see bel…

    Promoter

    SFFV (spleen focus forming virus)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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