We narrowed to 4,214 results for: Bre;

1. pLenti-VQRRA-P2A-Puro

   Plasmid

   #110851

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. tet-pLKO.1_puro_shJUND #1

   Plasmid

   #136581

   Purpose

   Expresses an inducible short hairpin targeting human JUND sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shJUNDv1 (JUND Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT 3xFLAG ID2

   Plasmid

   #98390

   Purpose

   Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   TRE tight promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

   Plasmid

   #46941

   Purpose

   Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with TALEN TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   embedded TALEN TS from 163-218, truncated 25 amin…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBrain-GFP-TACC3KDP(S558A)-shTACC3

   Plasmid

   #59357

   Purpose

   Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-SVOP [N356/23R]

   Plasmid

   #114499

   Purpose

   Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-SCG10/Stathmin-2 [L5/1R]

   Plasmid

   #140065

   Purpose

   Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_BRD003-sgSNAI1-2097

   Plasmid

   #115453

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgSNAI1-2097 (SNAI1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

12. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pEN_hU6miR-G12-E

    Plasmid

    #25757

    Purpose

    Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 12 miR-shRNA (Gna12 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-Pan-FHF-A [N235/22R]

    Plasmid

    #114509

    Purpose

    Mammalian Expression Plasmid of anti-Pan-FHF-A (Human). Derived from hybridoma N235/22.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Pan-FHF-A (Homo sapiens) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-HF1-PGK-Puro

    Plasmid

    #110857

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4

    Plasmid

    #112844

    Purpose

    transient/stable overexpression of the N-terminal and central domains of Catulin.

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Catulin-alpha-1 (Ctnnal1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N + C1 domains of Catulin

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-Pannexin-2 [N121A/31R]

    Plasmid

    #140073

    Purpose

    Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

    Plasmid

    #113040

    Purpose

    AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TTmiRc2_BirA_CDKN1A

    Plasmid

    #136523

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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