We narrowed to 13,220 results for: CRISPR-Cas9

1. pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101112

   Purpose

   Encodes dCas9(N) fused to AVPR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   AVPR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101116

   Purpose

   Encodes dCas9(N) fused to LPAR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   LPAR1-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-Terminal Split Cas9 D10A Nickase with GyrA intein

   Plasmid

   #58695

   Purpose

   Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Nsplit Intein, HA Tag, and NLS

   Expression

   Mammalian

   Mutation

   D10A nickase converting mutation to SpCas9

   Promoter

   CBh

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-SauriCas9-KKH-Puro

    Plasmid

    #135966

    Purpose

    Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1a

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-cmv-SpCas9-TadCBEd-BlastR

    Plasmid

    #193848

    Purpose

    Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-cmv-SpCas9-TadCBEa-BlastR

    Plasmid

    #193847

    Purpose

    Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV.U6.gDNMT1.Cas9-T2A-GFP

    Plasmid

    #170362

    Purpose

    Plasmid used for Crispr/cas9 based disruption of human DNMT1.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSH231-EF1-BLST-Cas9-VPR

    Plasmid

    #115147

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-Cas9-VPR
    

    Use

    CRISPR

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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