We narrowed to 12,189 results for: LEA
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Plasmid#31478DepositorInsertSFFV HA NLS Y2 K227M T2A BFP
UseLentiviralTagsHA and NLSMutationF13Y, S111Y, K227M = Y2 nickase "reo" …Available sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-KASH2ext
Plasmid#187018PurposeControl for inducible disruption of LINC ComplexDepositorInsertKASH2ext
TagsGFPExpressionMammalianAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-T3-hCASeGFP-pA
Plasmid#59766PurposeExpresses CAS9-eGFP fusion protein in mammalian cells.DepositorPublicationInsertCAS9
TagsFlag and eGFPExpressionMammalianPromoterCAGAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Synthetic, Human)
Tags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
TagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SS-HA-Sun1L-KDEL
Plasmid#187016PurposeDisruption of LINC ComplexDepositorInsertSS-HA-Sun1L-KDEL
UseLentiviralAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY0738 pCAG-NLS-SscINT
Plasmid#179113PurposeSscINT expression vectorDepositorInsertpCAG-NLS-SscINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0739 pCAG-NLS-SacINT
Plasmid#179114PurposeSacINT expression vectorDepositorInsertpCAG-NLS-SacINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)
Viral prep#112677-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-Tau-cYFP
Plasmid#92205PurposeRetroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)DepositorAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-nYFP
Plasmid#92204PurposeRetroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)DepositorAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-DroshaS300/302A
Plasmid#62530PurposeSerines 300 and 302 of Drosha are mutated to alaninesDepositorInserthuman Drosha with serines 300 and 302 changed alanines (DROSHA Human)
TagsGFPExpressionMammalianMutationchanged serines 300 and 302 to alaninesAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1
Plasmid#169449Purposeexpression of TRF1-mcherrry and sgRNA targeting telomeresDepositorInsertmCherry-TRF1
UseLentiviralExpressionMammalianAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorPublicationInsertTn5
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1h
Plasmid#190473PurposeExpress the high-affinity histamine sensor GRAB_HA1h in neuronsDepositorInsertHistamine sensor GRAB_HA1h (high affinity)
UseAAVPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only