We narrowed to 5,827 results for: pcas

1. pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

   Plasmid

   #108848

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

   Plasmid

   #108846

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

   Plasmid

   #100979

   Purpose

   Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

   Depositor

   Heinrich Leonhardt

   Article

   Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

   Insert

   c-Myc NLS-FKBP-Venus N-T2A-mRFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

   Plasmid

   #108737

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

   Plasmid

   #197505

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

   Promoter

   CMV and T7

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

   Plasmid

   #87560

   Purpose

   Expresses aa397-423 of Flp recombinase fused to PYL CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO aa397-423
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   PYL

   Expression

   Mammalian

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

   Plasmid

   #87559

   Purpose

   Expresses aa1-396 of Flp recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO aa1-396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

   Plasmid

   #108773

   Purpose

   Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment N-terminus to aa269
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

   Plasmid

   #108774

   Purpose

   Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment aa270 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

    Plasmid

    #108840

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

    Plasmid

    #108836

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

    Plasmid

    #108842

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid

    #107323

    Purpose

    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #128167

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

    Plasmid

    #36879

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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