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MBD-IFP2.0C

Plasmid

#215748

Purpose

Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BZLF2

Plasmid

#195784

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF2 (BZLF2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNALP

Plasmid

#195817

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNALP

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNALP (EBNA-LP Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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KG#901

Plasmid

#110936

Purpose

Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SAR1-no stop

Plasmid

#67345

Purpose

hSar1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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hDAGLb-V5

Plasmid

#87677

Purpose

Expresses human DAGLb protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biochemistry. 2016 May 17;55(19):2713-21

Insert

Human Diacylglycerol Lipase Beta (DAGLB Human)

Tags

V5 tag

Expression

Mammalian

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP6

Plasmid

#158357

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP6 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSecTag-T210A-ATX

Plasmid

#17840

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

T210A-ATX (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
SpyTag003-OspC

Plasmid

#159998

Purpose

Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag003-OspC (20-209) C129S

Tags

His6-tag and Thrombin cleavage site

Expression

Bacterial

Mutation

C129S mutation improves expression level.

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Article

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pYPQ230-E795L

Plasmid

#176890

Purpose

Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

Depositor

Yiping Qi

Article

Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

Insert

LbCas12a-E795L

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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LD101: pMVP (L3-L2) eCFP + WPRE

Plasmid

#121775

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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