We narrowed to 17,992 results for: NSI
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Plasmid#110405PurposeCRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistanceDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only
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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlga
Plasmid#140292PurposeExpress soluble His-tagged zebrafish Kitlga in insect cellsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
RA35
Plasmid#141126PurposeExpresses dimeric LacI repressor (LacI-11) under Rhl promoterDepositorInsertsTagsssrA degradation tagExpressionBacterialMutationtruncated/dimeric version of LacI (missing last 1…Promoterengineered rhl promoter (responds to C4-HSL) and …Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE P199A
Plasmid#139427PurposeGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged proline 199 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE I137A
Plasmid#139319PurposeGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged isoleucine 137 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA105
Plasmid#128521PurposeExpresses amyloid human Lysozyme in Pichia pastorisDepositorAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-Ib
Plasmid#121896PurposePlasmid for making zebrafish apoA-Ib in situ probeDepositorInsertApoA1b (apoa1b Zebrafish)
Available SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBb.2
Plasmid#121899PurposePlasmid for making zebrafish apoBb.2 in situ probeDepositorInsertApoBb.2 (apobb.2 Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.3
Plasmid#121905PurposePlasmid for making zebrafish apoA-IVb.3 in situ probeDepositorInsertapoa4b.3 (apoa4b.3 Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChromeT-GFP
Plasmid#123316PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterSynAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVa
Plasmid#121902PurposePlasmid for making zebrafish apoA-IVa in situ probeDepositorInsertApoA4a (apoa4a Zebrafish)
UseUnspecifiedAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-Ia
Plasmid#121895PurposePlasmid for making zebrafish apoA-Ia in situ probeDepositorInsertapoA-Ia (apoa1a Zebrafish)
Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
Tags6His-TEVExpressionBacterialPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 BICC FL
Plasmid#112998PurposeFor protein expression and purification of full-length Drosophila BicCDepositorInsertfull length BicC (BicC Fly)
ExpressionBacterialAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgAMDHD1
Plasmid#102316Purposegenetic depletion of AMDHD1DepositorAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_TBG1
Plasmid#111696PurposeMAC-tagged gene expressionDepositorInsertTBG1 (TUBG1 Human)
ExpressionMammalianAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_COX14
Plasmid#111663PurposeMAC-tagged gene expressionDepositorInsertCOX14 (C12orf62 Human)
ExpressionMammalianAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_AKIP
Plasmid#111660PurposeMAC-tagged gene expressionDepositorInsertAKIP (AURKAIP1 Human)
ExpressionMammalianAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_MGST3
Plasmid#111670PurposeMAC-tagged gene expressionDepositorInsertMGST3 (MGST3 Human)
ExpressionMammalianAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166
Plasmid#108474Purposeexpression of vsv-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
TagsVSVExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Mis12-dCEN-Incenp
Plasmid#108477Purposeexpression of vsv-Mis12-dCEN-INCENPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
TagsVSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-2/3
Plasmid#91551PurposeProtein expression and purification of human SH3 domain construct BZRAP1-2/3DepositorInsertBZRAP1-2/3 (TSPOAP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-2/3
Plasmid#91557PurposeProtein expression and purification of human SH3 domain construct SH3RF2-2/3DepositorInsertSH3RF2-2/3 (SH3RF2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOXO1-2/2
Plasmid#91558PurposeProtein expression and purification of human SH3 domain construct NOXO1-2/2DepositorInsertNOXO1-2/2 (NOXO1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-3/3
Plasmid#91559PurposeProtein expression and purification of human SH3 domain construct BZRAP1-3/3DepositorInsertBZRAP1-3/3 (TSPOAP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP-2/2
Plasmid#91529PurposeProtein expression and purification of human SH3 domain construct GRAP-2/2DepositorInsertGRAP-2/2 (GRAP Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PIK3R2-1/1
Plasmid#91535PurposeProtein expression and purification of human SH3 domain construct PIK3R2-1/1DepositorInsertPIK3R2-1/1 (PIK3R2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK2-1/3-SV1/2
Plasmid#91538PurposeProtein expression and purification of human SH3 domain construct NCK2-1/3-SV1/2DepositorInsertNCK2-1/3-SV1/2 (NCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MPP4-1/1-IS3/3
Plasmid#91519PurposeProtein expression and purification of human SH3 domain construct MPP4-1/1-IS3/3DepositorInsertMPP4-1/1-IS3/3 (MPP4 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-1/3
Plasmid#91487PurposeProtein expression and purification of human SH3 domain construct BZRAP1-1/3DepositorInsertBZRAP1-1/3 (TSPOAP1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCF2-2/2
Plasmid#91488PurposeProtein expression and purification of human SH3 domain construct NCF2-2/2DepositorInsertNCF2-2/2 (NCF2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCF1-2/2
Plasmid#91492PurposeProtein expression and purification of human SH3 domain construct NCF1-2/2DepositorInsertNCF1-2/2 (NCF1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TJP2-1/1
Plasmid#91493PurposeProtein expression and purification of human SH3 domain construct TJP2-1/1DepositorInsertTJP2-1/1 (TJP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RIMBP2-2/3
Plasmid#91468PurposeProtein expression and purification of human SH3 domain construct RIMBP2-2/3DepositorInsertRIMBP2-2/3 (RIMBP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only