We narrowed to 41,777 results for: REP
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Plasmid#75478Purposemammalian expression of GADD34DepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterhUbCAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPKR-FLAG-IG
Plasmid#101792PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PKRDepositorAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
SuperNova/pRSETB
Plasmid#53234Purposemonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertmonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AIO-Puro
Plasmid#74630PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsPuromycin resistant markerExpressionMammalianPromoterCbhAvailable SinceMay 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Human, Synthetic)
TagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE
Plasmid#67291PurposeInduce human iN conversion from fibroblastDepositorAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -1000 to -1Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-PQRv3-RFP
Plasmid#73951PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.DepositorInsertssfGFP (superfolder GFP)
RFP
TagsA residual PQR peptide wii be left at the C-termi…ExpressionMammalianPromoterChicken beta actin and Chicken beta actin (shared…Available SinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Sec61B-V5-APEX2
Plasmid#83411PurposeExpressed C-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertSec61B-V5-APEX2 (SEC61B Human, Synthetic)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRubiG-T2A-Cas9
Plasmid#75348PurposeUbiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsGFP (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#73211Purposeexpress Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)DepositorInsertAAP2
UseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRubiC-T2A-Cas9
Plasmid#75347PurposeUbiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsmCherry (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FOS
Plasmid#79511PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -