We narrowed to 11,745 results for: pcDNA

1. pCDNA4.TO-ORF61-2xCSTREP

   Plasmid

   #136221

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF61
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_mGFP-FGFR3-K650E

   Plasmid

   #191757

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>G (p.K650E) + silent mutation c.1938C&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Hyg-mEGFP

   Plasmid

   #191847

   Purpose

   Expresses the monomeric form of EGFP

   Depositor

   Bertrand Collet

   Article

   Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSlo2.1 Slick/pCDNA3

   Plasmid

   #197317

   Purpose

   Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

   Depositor

   Christopher Lingle

   Insert

   Slo2.1 (Kcnt2 Mouse)
   

   Use

   Tags

   7xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-sACE2v2.4-IgG1

   Plasmid

   #149668

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/mCherry-pBirA-HA

   Plasmid

   #154458

   Purpose

   expresses mCherry-pBirA-HA/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry-pBirA-HA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F, T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-FNTB-HA

   Plasmid

   #86869

   Purpose

   To express FNTB with a HA tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTB (FNTB Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GLI1_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176968

   Purpose

   Mammalian expression vector encoding GLI1 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-TORCAR-NES

   Plasmid

   #175015

   Purpose

   Cytosol-targeted TORCAR.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   TORCAR-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1/HisC 14-3-3 epsilon wt

    Plasmid

    #11942

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

13. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Use

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 PA-mCit-BAD

    Plasmid

    #113459

    Purpose

    ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 associated agonist of cell death (BAD Human)
    

    Use

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-sACE2v2-sfGFP

    Plasmid

    #145173

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 ALK4 K234R

    Plasmid

    #80881

    Purpose

    mammalian expression of ALK4 K234R

    Depositor

    Aristidis Moustakas, Peter ten Dijke

    Article

    ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K234R

    Promoter

    CMV

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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